Erwin Chargaff

My name is Erwin Chargaff and I discovered the structure of desoxyribonucleic acid. After reading Oswald Averys report, in 1944, well-nigh how genes were composed of deoxyribonucleic acid I become motivate to begin work on the chemistry of nucleic acids. I started researching with the belief that DNA from different species are different so I had to come up with an experiment to examine the DNA from different species. It was hard for me because I couldnt get large amounts of DNA so it took awhile for my experimenting. After about 2 years I was qualified to come up with a solid purpose containing three steps.First, I had to separate the DNA motley with chromatography paper. Then I converted the separated components into mercury salts and last alone not least I was able to identify the purines and pyrimidines ( the building blocks of nucleic acids) by using their ultraviolet absorption spectra. I tested this method several(prenominal) times and was finally able to use it to examine the DNA of barm and pancreatic cells. About a month after, I submitted two text file on my findings of the DNA of calf thymus and beef spleen and eminence bacilli and yeast.My papers were rejected so I decided to improve my procedure and by doing that I was able to examine more species. I summarized what I found on nucleic acids in a review and finally in 1950, my experimenting lead to me discover important facts. I came up with three rules that I named as Chargaffs rule. The first rule is that the number of Adenine unceasingly equals the number of thymine. The second is that the number of guanine always equals the number of degree Celsius and the last one is that the purines (adenine and guanine) always equal the number of pyrimidines (thymine and cytosine.I was also able to prove that the DNA of various species are different from one another. I did not collaborate with anyone for my experiment but two years later I explained my findings to Watson and twirl who were then lat er to come up the with DNA structure. I was a bit surprised that I wasnt include in the Noble Prize since I did share my information with Watson and Crick and with that they were able to come up with the model. So, after Watson and Crick won the Nobel Prize, I withdrew from left my lab and started to write essays and lectures.